Biotechnology Specialist, Molecular Biologist, 4 years
I am looking for a challenging job as Molecular Biologist or R&D specialist
Biotechnology Specialist, Molecular Biologist - ( 2011 - now) PureBiologisc Sp.z o.o., WrocÅaw, Lower Silesian (Poland) Adjusted, modified, and developed protocols for experiments. Planned and performed new experiments according to the needs of each project. Recorded data, observations, conclusions and result to use them in presentations and publications. Performed tests: selected testing objectives, determined statistical design and strategy, evaluated and communicated results and finally advised to the company. Developed analytical tests and estimations on different materials such as raw materials and finished products. Used instrumentation such as FTIR, HPLC and UV. Presented conclusions and created documentation for reports in all areas of the laboratory. Arranged experiments as needed. Organized and supervised experiment data. Designing gene construct for protein expression and purification using analytical and scientific software. Analyzing the professional literature to solve scientifical problems.
Uniwersity of WrocÅaw - 2012 WrocÅaw, Lower Silesian, Poland Medical Biotechnology, Master of Biotechnology Science Master thesis tittle: Optimization of HER2 extracellular domain expression and purification.
In the diploma work, I planned to overexpress recombinant ECD domain of HER2 protein in mammalian expression platform. I designed genetic constructs of HER2-ECD variants containing 6xhistidine tag and antibody Fc fragment to facilitate purification. I optimized transfection and expression culture conditions using HEK293/EBNA and CHO-S cell lines. Culture medium secreted proteins were purified by Ni2+ chromatography and protein A affinity. As a result of conducted experiments, I obtained expression constructs and established protocols for the production of HER2 protein in a small scale.
Uniwersity of WrocÅaw - 2010 WrocÅaw, Lower Silesian, Poland Biotechnology, Bachelor Thesis tittle: Immobilization on nanoparticles methods.
DNA/RNA isolation, very good, 4 years
PCR reactions, very good, 3 years
molecular cloning, good, 3 years
protein expression, good, 3 years
protein refolding, good, 3 years
protein purification, good, 3 years
Western Blotting, good, 2 years
DNA and protein sequence analysis, good, 3 years
capacity to work in a team environment, very good, 4 years